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Optical tweezers are unique tools for studying biophysical properties of single molecules. Design and construction of optical tweezers are very important. The optical path, the radial manipulation equation and axial manipulation equation of optical tweezers based on a finite conjugate microscope system are calculated using matrix optics. The influences of axial position adjustment of the objective, the installation location error of the coupling lens, the installation location error of the laser beam control system, and the installation location error of the confocal system lens' on radial trap position manipulation accuracy and axial trap position manipulation accuracy are analyzed. The results show that axial position adjustment of objective introduces no error in radial and axial trap position manipulation. The misalignment of laser beam control system has no effect on the radial manipulation, nor on axial manipulation when the coupling lens maintains alignment. It is concluded that misalignment of components of optical tweezers based on a finite conjugate microscope system has a greater effect on trap position manipulation error than misalignment of components of optical tweezers based on a infinite conjugate microscope system. The radial trap position manipulation error is less than 5.9% and the axial trap position manipulation error is less than 11.4% when the coupling lens installation location error is less than 10 mm. It is shown that optical tweezers can be modified from a finite conjugate microscope system. The formulations provide the basis for theoretical analysis of experimental alignment and adjustment.
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Keywords:
- optical tweezers /
- optical design /
- matrix /
- error
[1] Ashkin A, Dziedzic J M, Bjorkholm J E, Chu S 1986 Opt. Lett. 11 228
[2] Svoboda K, Schmidt C F, Schnapp B J, Block S M 1993 Nature 365 721
[3] Gross P, Laurens N, Oddershede L B, Bockelmann U, Peterman E J G, Wuite G J L 2011 Nat. Phys. 7 721
[4] Yasuharu A, Ryohei Y, Ken-ichirou A, Yoshie H, Hidetake M, Kazuhiko K J, Hiroyasu I 1999 Nature 399 446
[5] Dholakia K, Čižmár T 2011 Nat. Photon. 5 335
[6] Overton G 2009 Laser Focus World 45 17
[7] Vanzi F, Capitanio M, Sacconi L, Stringari C, Cicchi R, Canepari M, Maffei M, Piroddi N, Poggesi C, Nucciotti V, Linari M, Piazzesi G, Tesi C, Antolini R, Lombardi V, Bottinelli R, Pavone F S 2006 J. Muscle Res. Cell M 27 469
[8] Mishra Y N, Ingle N, Mohanty S K 2011 J. Biomed. Opt. 16 105003
[9] Kong L B, Zhang P F, Wang G W, Yu J, Setlow P, Li Y Q 2011 Nat. Protoc. 6 625
[10] Farre A, Marsa F, Montes-Usategui M 2012 Opt. Express 20 12270
[11] Valentine M T, Guydosh R, Gutierrez-Medina B, Fehr A N, Andreasson J O, Block S M 2008 Opt. Lett. 33 599
[12] Zhou J H, Tao R Z, Hu Z B, Zhong M C, Wang Z Q, Cai J, Li Y M 2009 Chin. Phys. Lett. 26 068701
[13] Jiang Y Q, Guo H L, Liu C X, Li Z L, Cheng B Y, Zhang D Z, Jia S T 2004 Acta Phys. Sin. 53 1721 (in Chinese) [降雨强, 郭红莲, 刘春香, 李兆霖, 程丙英, 张道中, 贾锁堂 2004 53 1721]
[14] Fällman E, Axner O 1997 Appl. Opt. 36 2107
[15] Zhao Y Z, Sun H Y, Song F H, Tang L M, Wu W W, Zhang X, Guo H C 2008 Acta Phys. Sin. 57 2284 (in Chinese) [赵延仲, 孙华燕, 宋丰华, 唐黎明, 吴伟伟, 张曦, 郭惠超 2008 57 2284]
[16] Ren H L, Ding P F, Li X Y 2012 Acta Phys. Sin. 61 210701 (in Chinese) [任洪亮, 丁攀峰, 李小燕 2012 61 210701]
[17] Lü B D 2003 Laser Optics Beam Characterization, Propagation and Transformation, Resonator Technology and Physics (3rd Ed.) (Beijing: Higher Education Press) p34 (in Chinese) [吕百达 2003 激光光学: 光束描述、传输变换与光腔技术物理 (第三版) (北京: 高等教育出版社) 第34页]
[18] Xu S H, Li Y M, Lou L R 2006 Chin. Phys. 15 1391
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[1] Ashkin A, Dziedzic J M, Bjorkholm J E, Chu S 1986 Opt. Lett. 11 228
[2] Svoboda K, Schmidt C F, Schnapp B J, Block S M 1993 Nature 365 721
[3] Gross P, Laurens N, Oddershede L B, Bockelmann U, Peterman E J G, Wuite G J L 2011 Nat. Phys. 7 721
[4] Yasuharu A, Ryohei Y, Ken-ichirou A, Yoshie H, Hidetake M, Kazuhiko K J, Hiroyasu I 1999 Nature 399 446
[5] Dholakia K, Čižmár T 2011 Nat. Photon. 5 335
[6] Overton G 2009 Laser Focus World 45 17
[7] Vanzi F, Capitanio M, Sacconi L, Stringari C, Cicchi R, Canepari M, Maffei M, Piroddi N, Poggesi C, Nucciotti V, Linari M, Piazzesi G, Tesi C, Antolini R, Lombardi V, Bottinelli R, Pavone F S 2006 J. Muscle Res. Cell M 27 469
[8] Mishra Y N, Ingle N, Mohanty S K 2011 J. Biomed. Opt. 16 105003
[9] Kong L B, Zhang P F, Wang G W, Yu J, Setlow P, Li Y Q 2011 Nat. Protoc. 6 625
[10] Farre A, Marsa F, Montes-Usategui M 2012 Opt. Express 20 12270
[11] Valentine M T, Guydosh R, Gutierrez-Medina B, Fehr A N, Andreasson J O, Block S M 2008 Opt. Lett. 33 599
[12] Zhou J H, Tao R Z, Hu Z B, Zhong M C, Wang Z Q, Cai J, Li Y M 2009 Chin. Phys. Lett. 26 068701
[13] Jiang Y Q, Guo H L, Liu C X, Li Z L, Cheng B Y, Zhang D Z, Jia S T 2004 Acta Phys. Sin. 53 1721 (in Chinese) [降雨强, 郭红莲, 刘春香, 李兆霖, 程丙英, 张道中, 贾锁堂 2004 53 1721]
[14] Fällman E, Axner O 1997 Appl. Opt. 36 2107
[15] Zhao Y Z, Sun H Y, Song F H, Tang L M, Wu W W, Zhang X, Guo H C 2008 Acta Phys. Sin. 57 2284 (in Chinese) [赵延仲, 孙华燕, 宋丰华, 唐黎明, 吴伟伟, 张曦, 郭惠超 2008 57 2284]
[16] Ren H L, Ding P F, Li X Y 2012 Acta Phys. Sin. 61 210701 (in Chinese) [任洪亮, 丁攀峰, 李小燕 2012 61 210701]
[17] Lü B D 2003 Laser Optics Beam Characterization, Propagation and Transformation, Resonator Technology and Physics (3rd Ed.) (Beijing: Higher Education Press) p34 (in Chinese) [吕百达 2003 激光光学: 光束描述、传输变换与光腔技术物理 (第三版) (北京: 高等教育出版社) 第34页]
[18] Xu S H, Li Y M, Lou L R 2006 Chin. Phys. 15 1391
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