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Bacillus subtilis, Aspergillus niger spore and Aspergillus niger mycelium are chosen for samples preparation. The above three samples' reflection spectra in 2.5—15 μm band are recorded by using infrared microscopic spectrometer. From Kramers-Kronig (K-K) relationship, microbe samples' complex refractive indexes in 6—14 μm band are calculated. According to the microbe samples' absorption spectra, their water contents are calculated using the absorption characteristics of water, protein and nucleic acid. Based on the above three microbe samples' water contents and complex refractive indexes in far infrared band, biologic particle's complex refractive index model in 6—14 μm band are constructed and the model's reliability is analyzed. The construction of biologic particle's complex refractive index model has important significance in the development of rapid analysis and identification method of biologic samples, and the detection and identification technologies of biologic aerosol.
[1] Nachman P, Chen G, Pinnick R G, Hill S C, Chang R K, Mayo M W, Fernandez G L 1996 Appl. Opt. 35 1069
[2] Dalterio R A, Nelson W H, Britt D, Sperry J, Psaras D, Tanguay J F, Suid S L 1986 Appl. Spectrosc. 40 86
[3] Dalterio R A, Nelson W H, Britt D, Sperry J, Tanguay J F, Suid S L 1987 Appl. Spectrosc. 41 234
[4] Bronk B V, Reinisch L 1993 Appl. Spectrosc. 47 436
[5] Pinnick R G, Hill S C, Nachman P 1995 Aerosol Science and Technology 23 653
[6] Feng C X, Huang L H, Zhou G C, Han J, Zeng A J, Zhao Y K, Huang H J 2010 Chinese Journal of Lasers 37 2593 (in Chinese) [冯春霞, 黄立华, 周光超, 韩杰, 曾爱军, 赵永凯, 黄惠杰 2010 中国激光 37 2593]
[7] Lan T G, Xiong W, Fang Y H, Li D C, Yuan Y M 2010 Acta Optica Sinica 30 1656 (in Chinese) [兰天鸽, 熊伟, 方勇华, 李大成, 袁越明 2010 光学学报 30 1656]
[8] Tuminello P S, Arakawa E T, Khare B N 1997 Appl. Opt. 36 2818
[9] Grosse P, Offermann V 1991 Appl. Phys. 52 138
[10] Booij H C, Thoone G P J M 1982 Rheol. Acta. 21 15
[11] Bohren C F, Huffman D R 1983 Absorption and scattering of light by small particles. (New York: John Wiley & Sons, Inc.) p228
[12] Bohren C F, Huffman D R 2009 Principles of Optics Electromagnetic Theory of Propagation, Inference and Diffraction of Light (Beijing: Publishing House of Electronics Industry) p32 (in Chinese)
[13] Dou Z W, Li X X, Zhao J J 2001 Acta ArmamentarII 32 498
[14] Rosenheimer M S, Linker R 2009 Journal of Quantitative Spectroscopy ＆ Radiative Transfer 110 1147
[15] Ross K F A, Billinge E 1957 J. Gen. Microbiol. 16 418
[16] Ci Y X, Zang K S, Gao T Y 2002 Chemical Journal of Chinese Universities 23 1047 (in Chinese) [慈云祥, 臧凯赛, 高体玉 2002 高等学校化学学报 23 1047]
[17] Li J W, Si M Z 2009 Chinese Journal of Spectroscopy Laboratory 26 888 (in Chinese) [李家旺, 司民真 2009 光谱实验室 26 888]
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[1] Nachman P, Chen G, Pinnick R G, Hill S C, Chang R K, Mayo M W, Fernandez G L 1996 Appl. Opt. 35 1069
[2] Dalterio R A, Nelson W H, Britt D, Sperry J, Psaras D, Tanguay J F, Suid S L 1986 Appl. Spectrosc. 40 86
[3] Dalterio R A, Nelson W H, Britt D, Sperry J, Tanguay J F, Suid S L 1987 Appl. Spectrosc. 41 234
[4] Bronk B V, Reinisch L 1993 Appl. Spectrosc. 47 436
[5] Pinnick R G, Hill S C, Nachman P 1995 Aerosol Science and Technology 23 653
[6] Feng C X, Huang L H, Zhou G C, Han J, Zeng A J, Zhao Y K, Huang H J 2010 Chinese Journal of Lasers 37 2593 (in Chinese) [冯春霞, 黄立华, 周光超, 韩杰, 曾爱军, 赵永凯, 黄惠杰 2010 中国激光 37 2593]
[7] Lan T G, Xiong W, Fang Y H, Li D C, Yuan Y M 2010 Acta Optica Sinica 30 1656 (in Chinese) [兰天鸽, 熊伟, 方勇华, 李大成, 袁越明 2010 光学学报 30 1656]
[8] Tuminello P S, Arakawa E T, Khare B N 1997 Appl. Opt. 36 2818
[9] Grosse P, Offermann V 1991 Appl. Phys. 52 138
[10] Booij H C, Thoone G P J M 1982 Rheol. Acta. 21 15
[11] Bohren C F, Huffman D R 1983 Absorption and scattering of light by small particles. (New York: John Wiley & Sons, Inc.) p228
[12] Bohren C F, Huffman D R 2009 Principles of Optics Electromagnetic Theory of Propagation, Inference and Diffraction of Light (Beijing: Publishing House of Electronics Industry) p32 (in Chinese)
[13] Dou Z W, Li X X, Zhao J J 2001 Acta ArmamentarII 32 498
[14] Rosenheimer M S, Linker R 2009 Journal of Quantitative Spectroscopy ＆ Radiative Transfer 110 1147
[15] Ross K F A, Billinge E 1957 J. Gen. Microbiol. 16 418
[16] Ci Y X, Zang K S, Gao T Y 2002 Chemical Journal of Chinese Universities 23 1047 (in Chinese) [慈云祥, 臧凯赛, 高体玉 2002 高等学校化学学报 23 1047]
[17] Li J W, Si M Z 2009 Chinese Journal of Spectroscopy Laboratory 26 888 (in Chinese) [李家旺, 司民真 2009 光谱实验室 26 888]
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