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Nano-resolution imaging based on single molecule localization is simulated with Matlab. A sample consisted of several lines of molecules are simulated imaged. The results demonstrate that two lines of molecules separated by 20 nm can be resolved. Effect of pixel size on the localization accuracy is also discussed here. Nano-resolution imaging is realized on an IX71 inverted fluorescent microscope with the resolution (FWHM) of 48 nm. Nano-resolution images of filopodias are reconstructed, in which filopodias with diameter of 75 nm are resolved.
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Keywords:
- nano-resolution /
- single molecule localization /
- HeLa cell /
- microtube /
- filopodia
[1] Wang C, Wang G Y, Xu Z M 2004 Acta Phys. Sin. 53 1325 (in Chinese) [王 琛、王桂英、徐至展 2007 53 1325]
[2] Liu L X, Qu J L, Lin Z Y, Chen D N, Xu G X, Hu T, Guo B P, Niu H B 2006 Acta Phys. Sin. 55 6281 (in Chinese) [刘立新、屈军乐、林子扬、陈丹妮、许改霞、胡 涛、郭宝平、牛憨笨 2006 55 6281]
[3] Dyba M, Jakobs S, Hell S W 2003 Nat. Biotechnol. 21 1303
[4] Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S, Davidson M W, Lippincott-Schwartz J, Hess H F 2006 Science 313 1642
[5] Rust M J, Bates M, Zhuang X 2006 Nat. Methods 3 793
[6] Cheng Y, Chen J F 2009 Laser Optoelectron.Prog. 46 35(in Chinese)[程 亚、陈建芳 2009 激光与光电子学进展 46 35]
[7] Zemer Gitai 2009 Curr. Opin. Microbio. 12 341
[8] Heilemann M, van de Linde S, Schüttpelz M, Kasper R, Seefeldt B, Mukherjee A, Tinnefeld P, Sauer M 2008 Angew. Chem. Int. Ed. 47 6172
[9] Thompson R E, Larson D R, Webb W W 2002 Biophy. J. 82 2775
[10] Yang C S, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T 2007 PLoS Bio. 5 2624
[11] Chen D, Yu B, Qu J, Niu H 2010 Opt. Lett. 35 886
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[1] Wang C, Wang G Y, Xu Z M 2004 Acta Phys. Sin. 53 1325 (in Chinese) [王 琛、王桂英、徐至展 2007 53 1325]
[2] Liu L X, Qu J L, Lin Z Y, Chen D N, Xu G X, Hu T, Guo B P, Niu H B 2006 Acta Phys. Sin. 55 6281 (in Chinese) [刘立新、屈军乐、林子扬、陈丹妮、许改霞、胡 涛、郭宝平、牛憨笨 2006 55 6281]
[3] Dyba M, Jakobs S, Hell S W 2003 Nat. Biotechnol. 21 1303
[4] Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S, Davidson M W, Lippincott-Schwartz J, Hess H F 2006 Science 313 1642
[5] Rust M J, Bates M, Zhuang X 2006 Nat. Methods 3 793
[6] Cheng Y, Chen J F 2009 Laser Optoelectron.Prog. 46 35(in Chinese)[程 亚、陈建芳 2009 激光与光电子学进展 46 35]
[7] Zemer Gitai 2009 Curr. Opin. Microbio. 12 341
[8] Heilemann M, van de Linde S, Schüttpelz M, Kasper R, Seefeldt B, Mukherjee A, Tinnefeld P, Sauer M 2008 Angew. Chem. Int. Ed. 47 6172
[9] Thompson R E, Larson D R, Webb W W 2002 Biophy. J. 82 2775
[10] Yang C S, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T 2007 PLoS Bio. 5 2624
[11] Chen D, Yu B, Qu J, Niu H 2010 Opt. Lett. 35 886
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