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Based on a spherically symmetric bubble model, the threshold shear stress for H-22 cells which are irradiated by continuous focused ultrasound is estimated. On the condition that the final concentration of the superparamagnetic iron oxide (SPIO) in the cell suspension is 410 g/mL, the focused ultrasound sonication is at a frequency of 1.37 MHz, the power from amplifer is 2 W, and H-22 cells are exposed to the ultrasound for 60 s, the labeling efficiency is about 45.9%13.5%. Prussian blue staining confirms iron uptake and shows numerous blue-stained iron particles in the cytoplasm, while more than 90% labeled cells remained viable. The results show that the reparable sonoporation arises in plasma membrane and SPIO nanoparticles enter into the H-22 cells. Numerical calculations show that the shear stress acting on the cell is 697 Pa, which is the threshold shear stress for H-22 cell sonoporation.
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Keywords:
- sonoporation /
- magnetic labeling /
- microstreaming /
- shear stress
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[38] Rooney A 1972 J. Acoust. Soc. Am.52 1718
[39] -
[1] Miller D L, Bao S, Morris J E 1999 Ultrasound Med. Biol. 25 143
[2] [3] Zhang C B, Qiu Y Y, Xi X Y, Zhang D 2009 Acta Phys. Sin. 58 3396(in Chinese) [张春兵、邱媛媛、郗晓宇、章 东 2009 58 3396]
[4] [5] Huber P E, Pfisterer P 2000 Gene. Ther. 7 1516
[6] [7] Wu J 2002 Ultrasound Med. Biol.28 125
[8] [9] Whelan J 2002 Drug Delivery Today 7 585
[10] Mo R Y, Wang G Z 2008 Acta Biophys. Sin. 24 445(in Chinese) [莫润阳、王公正 2008 生物 24 445]
[11] [12] Cheng J C, Liang B, Zhu Z M 2006 Chin. Phys. 15 412
[13] [14] Wu J, Nyborg W L 2008 Adv. Drug Deliver. Rev. 60 1103
[15] [16] [17] Wu J, Ross J R, Chiu J F 2002 J. Acoust. Soc. Am. 111 1460
[18] [19] Wamel A van, Kooiman K, Harteveld M 2006 J. Control Release 112 149
[20] Prenticel P, Cuschieri A, Dholakia K, Prausnitz M, Campbell P 2005 Nature Physics 1 107
[21] [22] [23] Ohl C D, Arora M, Lkink R, Jong N, Versluis M, Delius M, Lohse D 2006 Biophys. J. 91 4285
[24] Lewin P A, Leif Bjrn 1982 J. Acoust. Soc. Am.71 728
[25] [26] Wu E L, Wang P, Wang X B, Qi H, Liu Q H 2006 Appl. Acoust. 25 121(in Chinese) [吴二林、王 攀、王筱冰、齐 浩、刘全宏 2006 应用声学 25
[27] [28] 121 McNeil P L 1989 Methods Cell Biol. 29 153
[29] [30] [31] Gracewski S M, Miao H Y, Dalecki D 2005 J. Acoust. Soc. Am. 117 1140
[32] Chen Q, Zou X H, Cheng J C 2006 Acta Phys. Sin. 55 6476(in Chinese) [陈 谦、邹欣晔、程建春 2006 55 6476]
[33] [34] [35] Wu J 2007 Prog. in Biophys. and Mole. Biol. 93 363
[36] [37] Long M,Wu Z Z,Wang H B, Song G B,Wang X H,Wu Y P 1996 Acta Biophys. Sin.12 169(in Chinese) [龙 勉、吴泽志、王红兵、宋关斌、王宪航、吴云鹏 1996生物 12 169]
[38] Rooney A 1972 J. Acoust. Soc. Am.52 1718
[39]
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