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用316L不锈钢(SU316L)作基片,以高纯石墨作靶、CHF3和Ar作源气体,采用反应磁控溅射法在不同流量比R (CHF3／Ar)下制备了氟化类金刚石(F-DLC)薄膜. 利用双蒸水液滴法、BCA(二喹啉甲酸)法和傅里叶红外光谱(FTIR)探讨了影响薄膜蛋白吸附能力的因素. 结果表明,镀有F-DLC薄膜的SU316L表面的血小板黏附量明显减少,血小板的变形程度显著减轻,相应的白蛋白与纤维蛋白原的吸附比普遍高于未镀膜的SU316L表面的相应值,说明镀上F-DLC薄膜可以改善样品的血液相容性. 流量比为2 ∶1左右时制备出的F-DLC薄膜,其白蛋白与纤维蛋白原的吸附比值达到最大,相应的血液相容性最佳. 对薄膜的接触角和表面能以及FTIR光谱分析研究表明,SU316L表面的F-DLC薄膜的白蛋白与纤维蛋白原的吸附比及血液相容性与薄膜的中的-CFx键的含量(F/C比)和表面能(疏水性)直接相关,控制源气体流量比可以实现对薄膜的血液相容性的调制.The fluorinated diamond-like carbon (F-DLC) films are prepared by reactive magnetron sputtering under different gas flow radios with trifluoromethane (CHF3 ) and argon (Ar) used as source gases and pure graphite as a target on the surface of 316L stainless steel (SU316L). Factors which influence the protein adsorbability are discussed by double-stilled water, BCA and FTIR spectra. The results show that the surface of SU316L coated with F-DLC film could obviously reduce the number of adherent platelets and dramatically relieves the deformation of platelets, leading to a ratio of higher albumin to fibrinogen adsorption higher than that with using the SU316L substrates, which indicates that the SU316L coated with F-DLC film can improve the blood compatibility. The film has the highest ratio of albumin to fibrinogen adsorption and the best hemocompatibility when the ratio of gas flow is 2 ∶1. Furthermore, the measurements of the contact angle, the surface energy of films and FTIR spectra show that the ratio of albumin to fibrinogen adsorption and the hemocompatibility of F-DLC coated SU316L depend on the surface energy (hydrophobic nature) of films and the quantity of -CFx bonds (the ratio of F/C) contained in film. The modulating of blood compatibility of the films can be realized by the control of the ratio of source gas flow.
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Keywords:
- reactive magnetron sputtering /
- F-DLC films /
- proteins adsorbability
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[57] -
[1] Park J B, Kim Y K 2003 Biomaterials Principles and Applications(Boca Raton:CRC Press)p1
[2] Brunski J B 2004 Biomaterials Science an introduction to Materials in Medicine(San Diego:Elsevier Academic Press)p137
[3] [4] [5] Haidopoulos M, Turgeon S, Sarra-Bournet C 2006 Mater. Sci.: Mater. Med. 17 647
[6] [7] Yu Y T 2000 Bio-medical Materials (Tianjin:Tianjin University Press) p20 (in Chinese)[俞耀庭 2000 生物医用材料(天津:天津大学出版社)第20页]
[8] [9] Gorbet M B, Sefton M V 2004 Biomaterials 25 5681
[10] Armitage D A, Parker T L, Grant D M 2003 Biomed. Mater. Res. A 66 129
[11] [12] [13] Gutensohn K, Beythien C,Bau J, Fenner T,Grewe P,Koester R,Padmanaban K,Kuehnl P 2000 Thrombosis Res. 99 577
[14] [15] Ding M H,Wang B L, LI L, Zheng Y F 2010 Surf. Coat. Technol. 204 2519
[16] [17] Zhu L, Jiang M F, Ning Z Y, Du J L, Wang P J 2009 Acta Phys. Sin. 58 6430 (in Chinese)[朱 丽、江美福、宁兆元、杜记龙、王培君 2009 58 6430]
[18] Wang P J, Jiang M F, Xin Y, Du J L, Dai Y F 2010 Acta Phys. Sin. 59 8920 (in Chinese)[王培君、江美福、辛 煜、杜记龙、戴永丰 2010 59 8920]
[19] [20] Hakovirta M, He X M, Nastasi M 2000 J. Appl. Phy. 88 1456
[21] [22] [23] Terumitsu H, Satoshi Y, Aki K, Yuko O, Atsushi H, Koki T, Tetsuya S 2007 Biomed. Mater. Res. A 83 1192
[24] Goodman S L, Grasel T G, Cooper S L, Albrecht R M 1989 Biomed. Mater. Res. 23 105
[25] [26] Allen R D, Zacharski L R, Widirstky S T, Rosenstein R, Zaitlin L M, Burgess D R 1979 Cell Biol. 83 126
[27] [28] [29] Yu K, Chen Z T, Qiu J 1996 Biomed. Eng. 13 189 (in Chinese) [喻 凯、陈治涛、邱 静 1996 生物医学工程学杂志 13 189]
[30] Wen X J 1997 Biomed. Eng. 14 164 (in Chinese) [文学军 1997 生物医学工程杂志 14 164]
[31] [32] [33] Wang C H, Leng X G 1998 Foreign Medical Sciences (Biomedical Engineering Fascicle) 21 1 (in Chinese) [王传华、冷希岗 1998 国外医学:生物医学工程分册 21 1]
[34] [35] Whicher S J, Brash J L 1978 Biomed. Mater. Res. 12 181
[36] Dion I, Roques X, Baquey C, Baudet E, Cathalinat B B, More N 1993 Biomed. Mater. Eng. 3 51
[37] [38] [39] Jones M I, McColl I R, Grant D M, Paker K G, Paker T L 2000 Biomed. Mater. Res. 52 413
[40] Cui F Z, Li D J 2000 Surf. Coat. Technol. 131 481
[41] [42] [43] Li B G, Yin J, Na J J, Yin G F, Zheng C Q 2005 Biomed. Eng. 22 20 (in Chinese) [李伯刚、殷 杰、那娟娟、尹光福、郑昌琼 2005 生物医学工程学杂志 22 20]
[44] [45] Jiang M F, Ning Z Y 2006 Surf. Coat. Technol. 200 3682
[46] Jiang M F, Ning Z Y 2004 Acta Phys. Sin. 53 1588 (in Chinese) [江美福、宁兆元 2004 53 1588]
[47] [48] [49] Takada N, Shibagaki K, Kadota K, Oyama K I 2001 Vac.Sci.Technol. A 19 689
[50] [51] Wang X, Harris H R, Bouldin K, Temkin H, Gangopadhyay S 2000 J. Appl. Phys. 87 621
[52] [53] Yokomichi H 1999 J. Appl. Phys. 86 2468
[54] Huang S, Xin Y, Ning Z Y 2002 Acta Phys. Sin. 51 2635 (in Chinese) [黄 松、辛 煜、宁兆元 2002 51 2635]
[55] [56] Ishihara M, Kosaka T, Nakamura T, Tsugawa K, Hasegawa M, Kokai F, Koga Y 2006 Diamond Relat. Mater. 15 1011
[57]
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