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鉴于不同生理病理状态下组织复折射率实部的变化不大，传统光学相干层析（OCT）成像技术在分子特异性识别上存在先天不足.为此，本文提出了基于受激辐射信号的OCT成像方法，可在实现传统散射成像的同时，实现基于受激辐射信号的分子成像.在超高分辨率谱域OCT系统的基础上，通过增设光谱分光与调制抽运光支路，建立了基于单宽谱光源的抽运探测谱域OCT系统，详细推导了调制抽运下受激辐射信号的获取与成像公式.利用搭建的抽运探测谱域OCT系统，实现了瞬态受激辐射信号的相干探测.基于同时获取的受激辐射OCT信号和传统OCT信号，成功重构了氮化物粉末构建样品的基于受激辐射信号的分子对比OCT图像.Due to unnoticeable changes in complex refractive index of tissue under varied pathological and physiological states, the traditional optical coherence tomography (OCT) is deficient in molecular characterization. In this paper, the stimulated-emission based optical coherence tomography is proposed, which provides both molecular contrast and scattering contrast OCT imaging simultaneously. Based on the established ultra-high resolution spectral domain OCT system, a pump-probe spectral domain OCT system with a single wide-bandwidth light source is developed through an added modulated pump beam via spectrum splitting. In addition, the theory about the stimulated-emission signal and the image formulation under the modulated pump beam is presented. The coherent detection of the transient stimulated emission is realized by the developed pump-probe spectral domain OCT system. With the stimulated-emission OCT signal and the traditional OCT signal obtained at the same time, molecular contrast OCT images of the samples consisting of nitride powder are reconstructed successfully.
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Keywords:
- optical coherence tomography /
- stimulated emission /
- pump-probe detection /
- molecular specificity
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[5] Welzel J, Lankenau E, Birngruber R, Engelhardt R 1997 J. Am. Acad. Dermatol. 37 958
[6] Welzel J 2001 Skin Res. Technol. 7 1
[7] Fujimoto J G 2003 Nat. Biotechnol. 21 1361
[8] Adler D C, Chen Y, Huber R, Schmitt J, Connolly J, Fujimoto J G 2007 Nat. Photon. 1 709
[9] Liu L, Gardecki J A, Nadkarni S K, Toussaint J D, Yagi Y, Bouma B E, Tearney G J 2011 Nat. Med. 17 1010
[10] Yan X G, Shen Y, Pan C, Li P, Ding Z H 2016 Acta Phys. Sin. 65 024201 (in Chinese)[严雪过, 沈毅, 潘聪, 李鹏, 丁志华 2016 65 024201]
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[13] Yan Y Z, Ding Z H, Wang L, Shen Y 2013 Acta Phys. Sin. 62 164204 (in Chinese)[颜扬治, 丁志华, 王玲, 沈毅 2013 62 164204]
[14] Huang L M, Ding Z H, Hong W, Wang C 2012 Acta Phys. Sin. 61 023401 (in Chinese)[黄良敏, 丁志华, 洪威王川 2012 61 023401]
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[16] Liang Y M, Zhou D C, Meng F Y, Wang M W 2007 Acta Phys. Sin. 56 3246 (in Chinese)[梁艳梅, 周大川, 孟凡勇王明伟 2007 56 3246]
[17] Robles F E, Wilson C, Grant G, Wax A 2011 Nat. Photon. 5 744
[18] Rao K D, Choma M A, Yazdanfar S, Rollins A M, Izatt J A 2003 Opt. Lett. 28 340
[19] Applegate B E, Izatt J A 2006 Opt. Express 14 9142
[20] Desmond J, Shelton R L, Applegate B E 2010 Opt. Express 18 12399
[21] Kim W, Applegate B E 2015 Opt. Lett. 40 1426
[22] Yoo H, Kim J W, Shishkov M, Namati E, Morse T, Shubochkin R, McCarthy J R, Ntziachristos V, Bouma B E, Jaffer F A, Tearney G J 2011 Nat. Med. 17 1680
[23] Min W, Lu S, Chong S, Roy R, Holtom G R, Xie X S 2009 Nature 461 1105
[24] Wei L, Min W 2012 Anal. Bioanal. Chem. 403 2197
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