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频域光学相干层析系统中扫描机构定位精度、 机械抖动及样品移动会造成A扫描信号幅值和相位发生波动, 影响生物组织成像质量. 利用最小灰度差匹配、Lorentzian曲线极值拟合和谱域光程差补偿等方法对A 扫描信号进行幅值配准. 通过对A扫描信号相位分布特征的匹配实现相位差检测与配准. 通过求已配准的A 扫描复信号之差, 消除静态组织对血流成像的影响. 进行了人眼扫描实验, 有效提取了视网膜三维血流图像. 实验结果表明, 提出的幅值及相位配准方法大大减小了系统扫描精度、人眼跳动等因素对生物组织在体成像质量的影响. 快速、精确的相位配准方法也可广泛应用在多普勒OCT、相位显微等与相位分辨有关的光学成像领域.The amplitude and phase fluctuations of A-scans, influenced by the galvanometer positioning accuracy, mechanical jitter, and especially the ovement of the sample, may lead to the distortion and degradation of the tissue structure images and the blood flow images within tissue beds in frequency domain optical coherence tomography. The amplitude registration method for A-scans is proposed based on the minimum intensity difference matching criterion, Lorentzian curve fitting method, and compensation of the optical path difference in interference spectrum. The phase differences between A-scans are detected and registered by matching of phase distribution characteristics of A-scans. After the amplitude and phase registration, the complex signal differences between two A-scans are calculated to eliminate the effect of the static tissue on the blood flow imaging. The eye scanning experiments were performed and three-dimensional blood flow images of retina were acquired. Experiment results indicate that the effect of system scanning accuracy and tissue movement on the image quality can be effectively eliminated by the proposed amplitude and phase registration method. The fast and accurate phase registration method can also be used in Doppler OCT, phase microscope, and other phase related applications.
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Keywords:
- frequency domain optical coherence tomography /
- registration /
- blood flow imaging /
- phase distribution characteristics
[1] Shang Z M, Ding Z H, Wang L, Liu Y 2011 Acta Phys. Sin. 60 21404 (in Chinese) [商在明, 丁志华, 王玲, 刘勇 2011 60 21404]
[2] Shen T M, Gu Y 2007 Chin. J Laser Med. Surg. 16 379 (in Chinese) [沈婷梅, 顾瑛 2007 中国激光医学杂志 16 379]
[3] Kim D Y, Fingler J, Werner J S, Schwartz D M, Fraser S E, Zawadzki R J 2011 Biomed. Opt. Express 2 1504
[4] Kwiterovich K A, Maguire M G, Murphy R P 1991 Ophthalmology 98 1139
[5] Wang X J, Milner T E, Nelson J S 1995 Opt. Lett. 20 1337
[6] Chen Z, Milner T E, Dave D, Nelson J S 1997 Opt. Lett. 22 64
[7] Meng J, Ding Z H, Zhu Y 2009 Acta Optica Sinica 29 3168 (in Chinese) [孟婕, 丁志华, 朱瑛 2009 光学学报 29 3168]
[8] White B R, Pierce M C, Nassif N, Cense B, Park B H, Tearney G J, Bouma B E, Chen T C, Boer J F 2003 Opt. Express 11 3490
[9] Leitgeb R, Schmetterer L, Drexler W, Fercher A, Zawadzki R, Bajraszewski T 2003 Opt. Express 11 3116
[10] Zotter S, Pircher M, Torzicky T, Bonesi M, Götzinger E, Leitgeb R A, Hitzenberger C K 2011 Opt. Express 19 1217
[11] Makita S, Jaillon F, Yamanari M, Miura M, Yasuno Y 2011 Opt. Express 19 1271
[12] Fingler J, Schwartz D, Yang C H, Fraser S E 2007 Opt. Express 15 12636
[13] Mariampillai A, Standish B A, Moriyama E H, Khurana M, Munce N R, Leung M K, Jiang J, Cable A, Wilson B C, Vitkin I A, Yang V X 2008 Opt. Lett. 33 1530
[14] Makita S, Hong Y, Yamanari M 2006 Opt. Express 14 7821
[15] Wang R K, Hurst S 2007 Opt. Express 15 11402
[16] An L, Qin J, Wang R K 2010 Opt. Express 18 8220
[17] Szkulmowski M, Szkulmowska A, Bajraszewski T, Kowalczyk A, Wojtkowski M 2008 Opt. Express 16 6008
[18] Huang L M, Ding Z H, Hong W, Wang C 2012 Acta Phys. Sin. 61 023401 (in Chinese) [黄良敏, 丁志华, 洪威, 王川 2012 61 023401]
[19] Bachmann A H, Villiger M L, Blatter C, Lasser T, Leitgeb R A 2007 Opt. Express 15 408
[20] Ferguson R D, Hammer D X, Paunescu L A, Beaton S, Schuman J S 2004 Opt. Lett. 29 2139
[21] Kinkelder R D, Kalkman J, Faber D 2011 Invest. Ophthalmol. Vis. Sci. 52 3908
[22] Joo C, Akkin T, Cense B, Park B H, de Boer J F 2005 Opt. Lett. 30 2131
[23] Lin D, Jiang X, Xie F, Zhang W, Zhang L, Bennion I 2004 Opt. Express 12 5729
[24] Yaqoob Z, Choi W, Oh S, Niyom L, Park Y, Fang Y C, Dasari R R, Badizadegan K, Feld M S 2009 Opt. Express 17 10681
[25] Tomlins P H, Wang R K 2007 Meas. Sci. Technol. 18 3365
[26] Yang C, Wax A, Hahn M S, Badizadegan K, Dasari R R, Feld M S 2001 Opt. Lett. 26 1271
[27] Ralston T S, Marks D L, Carney P S, Boppart S A 2006 3rd IEEE International Symposium on Biomedical Imaging: Nano to Macro April 6-9, 2006 p578
[28] Yu L F, Rao B, Zhang J, Su J P, Wang Q, Guo S G, Chen Z P 2007 Opt. Express 15 7634
[29] Vakoc B J, Yun S H, de Boer J F, Tearney G J, Bouma B E 2005 Opt. Express 13 5483
[30] An L, Wang R K 2008 Opt. Express 16 11438
[31] Braaf B, Vermeer K A, Vienola K V, de Boer J F 2012 Opt. Express 20 20516
[32] Liu G Z, Wang R K 2013 Chin. Opt. Lett. 11 031701
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[1] Shang Z M, Ding Z H, Wang L, Liu Y 2011 Acta Phys. Sin. 60 21404 (in Chinese) [商在明, 丁志华, 王玲, 刘勇 2011 60 21404]
[2] Shen T M, Gu Y 2007 Chin. J Laser Med. Surg. 16 379 (in Chinese) [沈婷梅, 顾瑛 2007 中国激光医学杂志 16 379]
[3] Kim D Y, Fingler J, Werner J S, Schwartz D M, Fraser S E, Zawadzki R J 2011 Biomed. Opt. Express 2 1504
[4] Kwiterovich K A, Maguire M G, Murphy R P 1991 Ophthalmology 98 1139
[5] Wang X J, Milner T E, Nelson J S 1995 Opt. Lett. 20 1337
[6] Chen Z, Milner T E, Dave D, Nelson J S 1997 Opt. Lett. 22 64
[7] Meng J, Ding Z H, Zhu Y 2009 Acta Optica Sinica 29 3168 (in Chinese) [孟婕, 丁志华, 朱瑛 2009 光学学报 29 3168]
[8] White B R, Pierce M C, Nassif N, Cense B, Park B H, Tearney G J, Bouma B E, Chen T C, Boer J F 2003 Opt. Express 11 3490
[9] Leitgeb R, Schmetterer L, Drexler W, Fercher A, Zawadzki R, Bajraszewski T 2003 Opt. Express 11 3116
[10] Zotter S, Pircher M, Torzicky T, Bonesi M, Götzinger E, Leitgeb R A, Hitzenberger C K 2011 Opt. Express 19 1217
[11] Makita S, Jaillon F, Yamanari M, Miura M, Yasuno Y 2011 Opt. Express 19 1271
[12] Fingler J, Schwartz D, Yang C H, Fraser S E 2007 Opt. Express 15 12636
[13] Mariampillai A, Standish B A, Moriyama E H, Khurana M, Munce N R, Leung M K, Jiang J, Cable A, Wilson B C, Vitkin I A, Yang V X 2008 Opt. Lett. 33 1530
[14] Makita S, Hong Y, Yamanari M 2006 Opt. Express 14 7821
[15] Wang R K, Hurst S 2007 Opt. Express 15 11402
[16] An L, Qin J, Wang R K 2010 Opt. Express 18 8220
[17] Szkulmowski M, Szkulmowska A, Bajraszewski T, Kowalczyk A, Wojtkowski M 2008 Opt. Express 16 6008
[18] Huang L M, Ding Z H, Hong W, Wang C 2012 Acta Phys. Sin. 61 023401 (in Chinese) [黄良敏, 丁志华, 洪威, 王川 2012 61 023401]
[19] Bachmann A H, Villiger M L, Blatter C, Lasser T, Leitgeb R A 2007 Opt. Express 15 408
[20] Ferguson R D, Hammer D X, Paunescu L A, Beaton S, Schuman J S 2004 Opt. Lett. 29 2139
[21] Kinkelder R D, Kalkman J, Faber D 2011 Invest. Ophthalmol. Vis. Sci. 52 3908
[22] Joo C, Akkin T, Cense B, Park B H, de Boer J F 2005 Opt. Lett. 30 2131
[23] Lin D, Jiang X, Xie F, Zhang W, Zhang L, Bennion I 2004 Opt. Express 12 5729
[24] Yaqoob Z, Choi W, Oh S, Niyom L, Park Y, Fang Y C, Dasari R R, Badizadegan K, Feld M S 2009 Opt. Express 17 10681
[25] Tomlins P H, Wang R K 2007 Meas. Sci. Technol. 18 3365
[26] Yang C, Wax A, Hahn M S, Badizadegan K, Dasari R R, Feld M S 2001 Opt. Lett. 26 1271
[27] Ralston T S, Marks D L, Carney P S, Boppart S A 2006 3rd IEEE International Symposium on Biomedical Imaging: Nano to Macro April 6-9, 2006 p578
[28] Yu L F, Rao B, Zhang J, Su J P, Wang Q, Guo S G, Chen Z P 2007 Opt. Express 15 7634
[29] Vakoc B J, Yun S H, de Boer J F, Tearney G J, Bouma B E 2005 Opt. Express 13 5483
[30] An L, Wang R K 2008 Opt. Express 16 11438
[31] Braaf B, Vermeer K A, Vienola K V, de Boer J F 2012 Opt. Express 20 20516
[32] Liu G Z, Wang R K 2013 Chin. Opt. Lett. 11 031701
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