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For a quantitative analysis of the detection performance of wavelength surface plasmon resonance sensor after the introduction of absorbing thin films, a mathematical model was established for the wavelength SPR biosensor. Based on absorption theory of dispersive media, the distribution of electromagnetic field in each film outside the prism is calculated. The results show that, after introducing the absorbing thin films, electromagnetic field energy distribution in each layer out of the prism surfaces was changed, the metal film contribution on the resonance absorption was reduced, which increases the half wave width of SPR curve and the minimum reflection coefficient, so the optimal film thickness of metal film was also changed. Then the effect of colloidal gold and immunogold on SPR biosensor has been theoretically and experimentally verified. The result shows that, compared with the 48 nm gold film SPR sensor, the sensitivity increases 1.5 times when 45nm gold film is surface-modified with 10nm nano-gold.
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Keywords:
- surface plasmon resonance /
- biosensors /
- nano-gold /
- metal films
[1] Hong X, du D D, Qiu Z R, Zhang G X 2007 Acta Phys. Sin. 56 7219(in Chinese)[洪 昕、杜丹丹、裘祖荣、张国雄 2007 56 7219]
[2] Kooyman R P H,Kolkman H, Van Gent J, Greve J 1988 Anal. Chim. Acta 213 35
[3] Homola J, Yee S S, Gauglitz G 1999 Sens. Actuators B Chem. 54 3
[4] Okamoto T, Yamaguchi I 2003 J. Phys. Chem. B 107 10321
[5] Hansen W N 1968 J. Opt. Soc. Am. 58 380
[6] Zheng X H, Wang Y Q, Zhang Y 2009 Acta Phys. Sin. 58 8751(in Chinese)[郑显华、王瑛琪、张 岩 2009 58 8751]
[7] He L, Musick M D, Nicewarner S R, Salinas F G, Benkovic S J, Natan M J, Keating C D 2000 J. Am. Chem. Soc. 122 9071
[8] Hanning A, Roeraade J, Delrow J J, Jorgenson R C 1999 Sens. Actuators B 5425
[9] Lee C M, Liao C H, Chang L B, Chiao T L 1996 Jpn. J. Appl. Phys.35 5448
[10] Baba A, Park M K, Advincula R C, Knoll W 2002 Langmuir 18 4648
[11] Johnson P B, Christy R W 1972 Phys. Rev. B 6 4370
[12] Ekgasit S, Thammacharoen C, Knoll W 2004 Anal. Chem. 76 561
[13] Ekgasit S, Thammacharoen C, Yu F, Knoll W 2004 Anal. Chem. 75 2210
[14] Dobierzewska-Mozrzymas E, Wojcik J, Bieganski P, Pieciul E 1999 Vacuum 54 289
[15] Wang P H, Dai X H, Zhao X L, Song X P, Sun Z Q 2006 Acta Optica Sinica 261871 [王佩红、戴旭涵、赵小林、宋学萍、孙兆奇 2006光学学报 261871]
[16] Born M, Wolf E (Translated by Yang J S) 1978Principles of Optics (Beijing: Science Press). p182—190 (in Chinese)[玻 恩、沃尔夫、 杨葭孙译 1978光学原理(北京:科学出版社)第182—190页]
[17] Guo YN, Xue WR, Zhang WM 2009 Acta Phys. Sin. 58 4168(in Chinese)[郭亚楠、薛文瑞、张文梅 2009 58 4068]
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[1] Hong X, du D D, Qiu Z R, Zhang G X 2007 Acta Phys. Sin. 56 7219(in Chinese)[洪 昕、杜丹丹、裘祖荣、张国雄 2007 56 7219]
[2] Kooyman R P H,Kolkman H, Van Gent J, Greve J 1988 Anal. Chim. Acta 213 35
[3] Homola J, Yee S S, Gauglitz G 1999 Sens. Actuators B Chem. 54 3
[4] Okamoto T, Yamaguchi I 2003 J. Phys. Chem. B 107 10321
[5] Hansen W N 1968 J. Opt. Soc. Am. 58 380
[6] Zheng X H, Wang Y Q, Zhang Y 2009 Acta Phys. Sin. 58 8751(in Chinese)[郑显华、王瑛琪、张 岩 2009 58 8751]
[7] He L, Musick M D, Nicewarner S R, Salinas F G, Benkovic S J, Natan M J, Keating C D 2000 J. Am. Chem. Soc. 122 9071
[8] Hanning A, Roeraade J, Delrow J J, Jorgenson R C 1999 Sens. Actuators B 5425
[9] Lee C M, Liao C H, Chang L B, Chiao T L 1996 Jpn. J. Appl. Phys.35 5448
[10] Baba A, Park M K, Advincula R C, Knoll W 2002 Langmuir 18 4648
[11] Johnson P B, Christy R W 1972 Phys. Rev. B 6 4370
[12] Ekgasit S, Thammacharoen C, Knoll W 2004 Anal. Chem. 76 561
[13] Ekgasit S, Thammacharoen C, Yu F, Knoll W 2004 Anal. Chem. 75 2210
[14] Dobierzewska-Mozrzymas E, Wojcik J, Bieganski P, Pieciul E 1999 Vacuum 54 289
[15] Wang P H, Dai X H, Zhao X L, Song X P, Sun Z Q 2006 Acta Optica Sinica 261871 [王佩红、戴旭涵、赵小林、宋学萍、孙兆奇 2006光学学报 261871]
[16] Born M, Wolf E (Translated by Yang J S) 1978Principles of Optics (Beijing: Science Press). p182—190 (in Chinese)[玻 恩、沃尔夫、 杨葭孙译 1978光学原理(北京:科学出版社)第182—190页]
[17] Guo YN, Xue WR, Zhang WM 2009 Acta Phys. Sin. 58 4168(in Chinese)[郭亚楠、薛文瑞、张文梅 2009 58 4068]
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