Application of fluorescence spectra based on radial basis function neural network in identification of bacteria

Wu Hao; Zhu Tuo; Kong Yan; Chen Wei; Yang Jian-Lei

doi:10.7498/aps.59.2396

Abstract

The results of research on fluorescence spectra of three kinds of probiotic bacteria (lactobacillus acidophilus, streptococcus mutans and lactobacillus bulgaricus) show that the bacteria emit fluorescence when irradiated by ultraviolet light. The spectra are in the range of 300—650　nm with the excitation wavelength of 290　nm. Wavelet transform is used to compress the 150 groups of spectra data, and the number of data points in each group is reduced from 1341 to 168, which not only keeps the character of original spectra unchanged, but also improves the processing speed of neural network. Radial basis function neural network is applied for processing the compressed data. 40 groups of data of each strain are used to be trained, and the other 30 groups of data of which the kinds of bacteria are not given are used for prediction. The result shows that radial basis function neural network can identify the unknown bacterial strain accurately.

Keywords:

Authors and contacts

1.
江南大学理学院，无锡 214122

References

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[17]	］Research and Development Center of Feisi Science and Technology Products　 2005　Neural Networks and Realized by Matlab7 (Beijing： Publishing House of Electronics Industry) (in Chinese) ［飞思科技产品研发中心 2005 神经网络理论与Matlab7实现（北京：电子工业出版社）］
[18]	］Dong C H 2007 Matlab Neural Networks and Application(2nd Ed.) (Beijing： National Defense Industry Press)　 (in Chinese) ［董长虹 2007　Matlab神经网络与应用 (第二版) （北京：国防工业出版社）］
[19]	］Ma S M, Liu S D, Zhang Z Y, Fan G Q 2005 Spectrosc. Spectr. Anal.　25 874 (in Chinese) ［马书民、刘思东、张卓勇、范国强 2005 光谱学与光谱分析 25 874］
[20]	］Li Y B, Huang X Y, Sha M， Meng X S　2001 Chin. J. Chromat. 19 112 (in Chinese) ［李一波、黄小原、沙明、孟宪生 2001　色谱 19 112］
[21]	］Yu X H, Zhang Z Y, Ma Q， Fan G Q 2007 Spectrosc. Spectr. Anal. 27 481 (in Chinese) ［于晓辉、张卓勇、马群、范国强　2007 光谱学与光谱分析 27 481］
[22]	］Wang K， Zhang H， Chang S J， Shen J Y 2007 Acta Phys. Sin. 56 3613 (in Chinese) ［王凯、张会、常胜江、申金媛 2007 56 3613］
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Cited By

[1]	［1］Yang J， Pan X H， Zheng Y Y， Dong M Z, Xiao P， Ye Y W， Zhong W J　2000 Shanghai J. Prev. Med. 12 73 (in Chinese) ［杨隽、潘喜华、郑勇英、董妙珠、肖萍、叶于薇、仲伟鉴 2000 上海预防医学杂志 12 73］
[2]	［2］Li Q, Zhang S C, Zeng X Y， Zhang T T 2004 Food Res. Dev.　25 106 (in Chinese) ［李琴、张世春、曾晓燕、张铁涛 2004 食品研究与开发 25 106］
[3]	［3］Wei H， Liu C M， Cheng B C， Xu F 2007　Food Sci.　28 618 (in Chinese) ［魏华、刘春梅、程波财、徐峰　2007 食品科学 28 618］
[4]	［4］Hu X Z 2001 Shanghai Med. Pham. J. 22 356 (in Chinese) ［胡学智 2001 上海医药 22 356］
[5]	［5］Guo X H 2002 Foundation and Application of Probiotics (Beijing： Beijing Scientific and Technical Publishers) (in Chinese) ［郭兴华 2002 益生菌基础与应用 (北京：北京科学技术出版社)］
[6]	［6］Zhang G 2007 Lactic Acid Bacteria—Basis， Technology and Application (Beijing： Chemical Industry Press) (in Chinese) ［张刚 2007 乳酸细菌——基础、技术和应用（北京：化学工业出版社）］
[7]	［7］Zhang L, Tan B P， Mai K　S, Ai Q H， Zhang W B， Xu W 2008 Period. Ocean Univ. Chin. 38 225 (in Chinese) ［张玲、谭北平、麦康森、艾庆辉、张文兵、徐玮 2008　中国海洋大学学报　38 225］
[8]	［8］Li H B, Song X L, Wei S, Li Y， Lan P 2008 Ocean Limin Sin. 39 374 (in Chinese) ［李海兵、宋晓玲、韦嵩、李赟、兰萍 2008　海洋与湖沼 39 374］
[9]	［9］Liu Y, Song C Y, He W L, Luo X S, Lu J, Ni X W 2007 Acta Phys. Sin. 56 2962 (in Chinese) ［刘莹、宋春元、何文亮、骆晓森、陆建、倪晓武 2007 　56 2962］
[10]	］Liu Y, Ni X W　2009 Acta Phys. Sin. 58 3572 (in Chinese) ［刘莹、倪晓武 2009 　58 3572］
[11]	］Zhang J F, Hu S S 2007 Acta Phys. Sin. 56 713 (in Chinese) ［张军峰、胡寿松 2007 56 713］
[12]	］Cheng L, Ding X L, Liu Z G, Pan Q L, Chu X L, Feng S L 2007 Acta Phys. Sin. 56 6894 (in Chinese) ［程琳、丁训良、刘志国、潘秋丽、初学莲、冯松林 2007 56 6894］
[13]	］Chin R C, Mayo M W, Hallidy W, Salazar N, Cruce C 1996 SPIE　2836 158
[14]	］Stephan D， Jrg M L， Rainer R， Jürgen R 1998 Marine Chem. 62 137
[15]	］Héctor E G， Landulfo S J， Renato A Z, Marcos T T P 2003 J. Fluorensc. 13 489
[16]	］Elliott S， Lead J R， Baker A 2006 Anal. Chim. Acta 564 219
[17]	］Research and Development Center of Feisi Science and Technology Products　 2005　Neural Networks and Realized by Matlab7 (Beijing： Publishing House of Electronics Industry) (in Chinese) ［飞思科技产品研发中心 2005 神经网络理论与Matlab7实现（北京：电子工业出版社）］
[18]	］Dong C H 2007 Matlab Neural Networks and Application(2nd Ed.) (Beijing： National Defense Industry Press)　 (in Chinese) ［董长虹 2007　Matlab神经网络与应用 (第二版) （北京：国防工业出版社）］
[19]	］Ma S M, Liu S D, Zhang Z Y, Fan G Q 2005 Spectrosc. Spectr. Anal.　25 874 (in Chinese) ［马书民、刘思东、张卓勇、范国强 2005 光谱学与光谱分析 25 874］
[20]	］Li Y B, Huang X Y, Sha M， Meng X S　2001 Chin. J. Chromat. 19 112 (in Chinese) ［李一波、黄小原、沙明、孟宪生 2001　色谱 19 112］
[21]	］Yu X H, Zhang Z Y, Ma Q， Fan G Q 2007 Spectrosc. Spectr. Anal. 27 481 (in Chinese) ［于晓辉、张卓勇、马群、范国强　2007 光谱学与光谱分析 27 481］
[22]	］Wang K， Zhang H， Chang S J， Shen J Y 2007 Acta Phys. Sin. 56 3613 (in Chinese) ［王凯、张会、常胜江、申金媛 2007 56 3613］
[23]	］Zhang H P, Zhao J B, Yang Z L 2003 Comput. Modern. 99 600 (in Chinese) ［张海鹏、赵景波、杨占录 2003 计算机与现代化 99 600］

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Vol. 59, Issue 4 - 2010-02-20

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