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A bare quartz optical fiber is implanted in a microfluidic channel of polydimethylsiloxane (PDMS) substrate. Pumping the microfluid by a continuous wave laser with a wavelength of 532 nm along the fiber axis, the fluorescent spectra from the channel filled with lower refractive index (RI) dye solution are obtained. Due to the fact that the evanescent field of the pump beam is homogeneous around fiber, the fluorescent emission from the rim of fiber is uniform. It is found experimentally that the fluorescent emission intensity decreases with the axial distance of fiber, and the intensity is very sensitive to the RI of the dye solution and the dye concentration. For the dye solution with a large RI, the emitted fluorescent intensity attenuates along the fiber axis more obviously than that of the dye solution with a small RI. For the high dye concentration solution, the emitted fluorescent intensity attenuates along the fiber axis also more significantly than that of the low dye concentration solution. Therefore, it is possible to obtain a uniform fluorescence radiation along the fiber axis by selecting a suitably smaller RI and a lower dye concentration solution. The observed experimental phenomena are well explained based on the mechanism of evanescent wave pumping fluorescent radiation. Based on the features of fluorescent emission in the microfluidic chip, a PDMS chip with three micro-channels is designed and fabricated. After injecting ethanol solutions of rhodamine 640, rhodamine B and rhodamine 6 G separately into the three channels and pumpingthese solutions by evanescent wave along the optical fiber axis, three fluorescence emissions with different wavelength ranges are successfully observed in a single PDMS chip.
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Keywords:
- microfluidic chip /
- fluorescence radiation characteristics /
- evanescent wave /
- tri-band fluorescent light sources
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[7] Chen Y C, Chen Q S, Fan X 2016 Lab on Chip 16 2228
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[10] Fan X, Yun S H 2014 Nat. Methods 11 141
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[18] Zhu K, Zhou L, You H H, Jiang N, Pu X Y 2011 Acta Phys. Sin. 60 054205 (in Chinese) [祝昆, 周丽, 尤洪海, 江楠, 普小云 2011 60 054205]
[19] Ulrich B 1986 Lambda Chrome Laser Dyes (Lambda: Lambda Physik Gmbh)
[20] Mayers B T, Vezenov D V, Vullev V I, Whitesides G M 2005 Anal. Chem. 77 1310
[21] Sun Y, Shopova S I, Wu C S, Amold S, Fan X D 2010 Proc. Natl. Acad. Sci. USA 107 16039
[22] Pilgyu K, Perry S, Xavier S, Dakota O D, David E 2015 Sci. Reports 5 12087
[23] Fan X D, White I M 2011 Nat. Photon. 5 591
[24] Mellors J S, Jorabchi K, Smith L M, Ramsey M 2010 Anal. Chem. 82 967
[25] Wu D, Luo Y, Zhou X M, Dai Z P 2005 Electrophoresis 26 1
[26] Vasdekis A E, Laporte G P J 2013 Int. J. Mol. Sci. 12 8
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[1] Thorsen T, Maerkl S J, Quake S R 2002 Science 298 580
[2] Liu K K, Wu R G, Chuang Y J, Khoo H S, Huang S H, Tseng F G 2010 Sensors 10 6623
[3] Manz A, Graber N, Widmer H 1990 Sensors and Actuators B: Chemical 1 244
[4] Psaltis D, Quake S R, Yang C 2006 Nature 442 27
[5] Helbo B, Kristensen A, Menon A 2003 J. Micromech. Microengin. 13 2
[6] Monat C, Domachuk P, Eggleton P B 2007 Nat. Photon. 1 106
[7] Chen Y C, Chen Q S, Fan X 2016 Lab on Chip 16 2228
[8] Gilardi G, Beccherelli R 2013 J. Phys. D: Appl. Phys. 46 105104
[9] Li M, Zhi M, Zhu H, Wu W Y, Xu Q H, Jhon M H, Chan Y 2015 Nat. Commun. 6 1
[10] Fan X, Yun S H 2014 Nat. Methods 11 141
[11] Zhang J, Wang S, Liu K, Wei Y, Wang X, Duan Y 2015 Anal. Chem. 87 2959
[12] Lim J M, Kim S H, Yang S M 2011 Microfluid. Nanofluid. 10 211
[13] Wolfe D B, Conroy R S, Garstecki P, Mayers B T, Fischbach M A, Paul M P, Whitesides G M 2004 Proc. Natl. Acad. Sci. USA 101 12434
[14] Vezenov D V, Mayers B T, Wolfe D B, Whitesides G M 2005 Appl. Phys. Lett. 86 041104
[15] Lim J M, Kim S H, Choi J H, Yang S M 2008 Lab on Chip 8 1580
[16] Moon H J, Chough Y T, An K 2000 Phys. Rev. Lett. 85 15
[17] Zhang Y X, Pu X Y, Zhu K, Feng L 2011 J. Opt. Soc. Am. B 28 2048
[18] Zhu K, Zhou L, You H H, Jiang N, Pu X Y 2011 Acta Phys. Sin. 60 054205 (in Chinese) [祝昆, 周丽, 尤洪海, 江楠, 普小云 2011 60 054205]
[19] Ulrich B 1986 Lambda Chrome Laser Dyes (Lambda: Lambda Physik Gmbh)
[20] Mayers B T, Vezenov D V, Vullev V I, Whitesides G M 2005 Anal. Chem. 77 1310
[21] Sun Y, Shopova S I, Wu C S, Amold S, Fan X D 2010 Proc. Natl. Acad. Sci. USA 107 16039
[22] Pilgyu K, Perry S, Xavier S, Dakota O D, David E 2015 Sci. Reports 5 12087
[23] Fan X D, White I M 2011 Nat. Photon. 5 591
[24] Mellors J S, Jorabchi K, Smith L M, Ramsey M 2010 Anal. Chem. 82 967
[25] Wu D, Luo Y, Zhou X M, Dai Z P 2005 Electrophoresis 26 1
[26] Vasdekis A E, Laporte G P J 2013 Int. J. Mol. Sci. 12 8
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