The recovery behavior of the 60% deformed iron in various stages of isochronal annealing has been investigated by positron annihilation technique. The experimental results show that both the average life time τ and the lineshape parameter S of the positron annihilation may be expressed as functions of annealing temperatures. Life time τf of the positron annihilated in the perfect lattice is found to be 111 ± 1 ps, while τd in the defect lattice to be 162 ± 1 ps. Trapping rates and τ1 are calculated, these values are in good agreement with those predicted by trapping theory. It is obtained from the calculation that the rang of fractional concentration of defect trapping positron is 10-7-10-4.