带电多孔二氧化硅纳米颗粒在硫醇/磷脂混合双层膜上的非特异性吸附

陆乃彦; 元冰; 杨恺

doi:10.7498/aps.62.178701

摘要

制备了表面带阴/阳离子的多孔二氧化硅纳米颗粒, 通过QCM-D研究了颗粒在不同pH值环境下与磷脂膜的非特异性吸附情况. 结果表明, NH2-MSN 在48的pH值范围内与磷脂膜相互吸引, 而COOH-MSN由于与磷脂膜的电性始终保持一致而无法发生吸附现象. 本研究能够帮助理解和预测纳米颗粒与细胞膜间的相互作用, 为药物输运提供载体, 有助于多孔二氧化硅纳米颗粒在药物输运体系中的应用.

关键词:

Mesoporous silica nanoparticles (MSN) with cationic and anionic surface charges were synthesized, Their adsorption behaviors to the supported lipid membranes at different pH values were also studied using QCM-D. We found that NH2-MSN could be adsorbed onto the membrane at pH values from 4 to 8, while the adsorption of COOH-MSN onto the membrane could not occur due to its charge being always the same as that of the membrane at any pH values. These results might provide the information for understanding and predicting the interactions between nanoparticles and cell membranes, and could be effectively used in drug delivery systems and disease treatment.

Keywords:

作者及机构信息

1.
南京大学物理学院, 固体微结构国家实验室, 南京 210093;

2.
苏州大学, 软凝聚态物理及交叉研究中心, 苏州 215006

基金项目: 国家自然科学基金(批准号: 11104192, 21106114);教育部科学技术研究重点项目(批准号: 210208)和云南省应用基础研究计划(批准号: 2010CD091)资助的课题.

Authors and contacts

1.
National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing 210093, China;

2.
Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, China

Funds: Project supported by the National Natural Science Foundation of China (Grant Nos. 11104192, 21106114), the Key Project of Chinese Ministry of Education, China (Grant No. 210208), and the Applied Basic Research Program of Yunnan Province, China (Grant No. 2010CD091).

参考文献

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[2]	Liu J W, Jiang X M, Ashley C, Brinker C J 2009 J. Am. Chem. Soc. 131 7567
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[8]	Liong M, Lu J, Kovochich M, Xia T, Ruehm S G, Nel A E, Tamanoi F, Zink J I 2008 ACS Nano. 2 889
[9]	Lei J M, L L, Liu L, Xu X L 2011 Acta Phys. Sin. 60 017501 (in Chinese) [雷洁梅, 吕柳, 刘玲, 许小亮 2011 60 017501]
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[11]	Cho E C, Xie J, Wurm P A, Xia Y 2009 Nano Lett. 9 1080
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[13]	Xia T, Kovochich M, Liong M, Zink J I, Nel A E 2008 ACS Nano. 2 85
[14]	Wilhelm C, Billotey C, Roger J, Pons J N, Bacri J C, Gazeau F 2003 Biomaterials 24 1001
[15]	Ryman-Rasmussen J P, Riviere J E, Monteiro-Riviere N A 2007 Nano Lett. 7 1344
[16]	Zhang X F, Yang S H 2011 Langmuir 27 2528
[17]	Rodahl M, Höök F, Fredriksson C, Keller C A, Krozer A, Brzezinski P, Voinova M, Kasemo B 1997 Faraday Discuss 107 229
[18]	Voinova M V, Jonson M, Kasemo B 2002 Biosensors and Bioelectronics 17 835
[19]	Richter R, Mukhopadhyay A, Brisson A 2003 Biophys. J 85 3035
[20]	Lu N Y, Yang K, Yuan B, Ma Y Q 2012 J. Phys. Chem. B 116 9432
[21]	Plant A L, Gueguechkeri M, Yap W 1994 Biophys. J. 67 1126
[22]	Plant A L 1999 Langmuir 15 5128
[23]	Jiang Z Y, Zhang G L, Ma J, Zhu T 2013 Acta Phys. Sin. 62 018701 (in Chinese) [蒋中英, 张国梁, 马晶, 朱涛 2013 62 018701]
[24]	Möller K, Kobler J, Bein T 2007 Adv. Funct. Mater. 17 605
[25]	Kecht J, Schlossbauer A, Bein T 2008 Chem. Mater. 20 7207
[26]	Cauda V, Engelke H, Sauer A, Arcizet D, Bräuchle C, Rädler J, Bein T 2010 Nano Lett. 10 2484
[27]	Park C, Oh K, Lee S C, Kim C 2007 Angew. Chem. Int. Ed. 46 1455
[28]	Academic A U 1991 Nature 354 120
[29]	Diao P, Jiang D L, Cui X L, Gu D P, Tong R T, Zhong B 1999 Bioelectrochem. Bioenerg. 48 469
[30]	Ding L, Li J H, Dong S J, Wang E K 1996 J. Electroanal. Chem. 416 105
[31]	Tu C K, Chen K, Tian W D, Ma Y Q 2013 Macromol. Rapid Comm. 34 1237
[32]	Ding H M, Ma Y Q 2012 Biomaterials 33 5798

施引文献

[1]	Yousaf M Z, Yu J, Hou Y L, Gao S 2013 Chin. Phys. B 22 058702
[2]	Liu J W, Jiang X M, Ashley C, Brinker C J 2009 J. Am. Chem. Soc. 131 7567
[3]	Giri S, Trewyn B G, Stellmaker M P, Lin V S Y 2005 Angew. Chem. Int. Ed. 44 5038
[4]	Hong C Y, Li X, Pan C Y 2008 J. Phys. Chem. C 112 15320
[5]	Liu R, Zhang Y, Zhao X, Agarwal A, Mueller L J, Feng P Y 2010 J. Am. Chem. Soc. 132 1500
[6]	Ashley C E, Carnes E C, Phillips G K, Padilla D, Durfee P N, Brown P A, Hanna T N, Liu J W, Phillips B, Carter M B, Carroll N J, Jiang X M, Dunphy D R, Willman C L, Petsev D N, Evans D G, Parikh A N, Chackerian B, Wharton W, Peabody D S, Brinker C J 2011 Nature Materials 10 389
[7]	Rosenholm J M, Peuhu E, Eriksson J E, Sahlgren C, Lindén M 2009 Nano Lett. 9 3308
[8]	Liong M, Lu J, Kovochich M, Xia T, Ruehm S G, Nel A E, Tamanoi F, Zink J I 2008 ACS Nano. 2 889
[9]	Lei J M, L L, Liu L, Xu X L 2011 Acta Phys. Sin. 60 017501 (in Chinese) [雷洁梅, 吕柳, 刘玲, 许小亮 2011 60 017501]
[10]	Kirchner C, Liedl T, Kudera S, Pellegrino T, Muñoz-Javier A, Gaub H E, Stölzle S, Fertig N, Parak W J 2005 Nano Lett. 5 2331
[11]	Cho E C, Xie J, Wurm P A, Xia Y 2009 Nano Lett. 9 1080
[12]	Arvizo R R, Miranda O R, Thompson M A, Pabelick C M, Bhattacharya R, Robertson J D, Rotello V M, Prakash Y S, Mukherjee P 2010 Nano Lett. 10 2543
[13]	Xia T, Kovochich M, Liong M, Zink J I, Nel A E 2008 ACS Nano. 2 85
[14]	Wilhelm C, Billotey C, Roger J, Pons J N, Bacri J C, Gazeau F 2003 Biomaterials 24 1001
[15]	Ryman-Rasmussen J P, Riviere J E, Monteiro-Riviere N A 2007 Nano Lett. 7 1344
[16]	Zhang X F, Yang S H 2011 Langmuir 27 2528
[17]	Rodahl M, Höök F, Fredriksson C, Keller C A, Krozer A, Brzezinski P, Voinova M, Kasemo B 1997 Faraday Discuss 107 229
[18]	Voinova M V, Jonson M, Kasemo B 2002 Biosensors and Bioelectronics 17 835
[19]	Richter R, Mukhopadhyay A, Brisson A 2003 Biophys. J 85 3035
[20]	Lu N Y, Yang K, Yuan B, Ma Y Q 2012 J. Phys. Chem. B 116 9432
[21]	Plant A L, Gueguechkeri M, Yap W 1994 Biophys. J. 67 1126
[22]	Plant A L 1999 Langmuir 15 5128
[23]	Jiang Z Y, Zhang G L, Ma J, Zhu T 2013 Acta Phys. Sin. 62 018701 (in Chinese) [蒋中英, 张国梁, 马晶, 朱涛 2013 62 018701]
[24]	Möller K, Kobler J, Bein T 2007 Adv. Funct. Mater. 17 605
[25]	Kecht J, Schlossbauer A, Bein T 2008 Chem. Mater. 20 7207
[26]	Cauda V, Engelke H, Sauer A, Arcizet D, Bräuchle C, Rädler J, Bein T 2010 Nano Lett. 10 2484
[27]	Park C, Oh K, Lee S C, Kim C 2007 Angew. Chem. Int. Ed. 46 1455
[28]	Academic A U 1991 Nature 354 120
[29]	Diao P, Jiang D L, Cui X L, Gu D P, Tong R T, Zhong B 1999 Bioelectrochem. Bioenerg. 48 469
[30]	Ding L, Li J H, Dong S J, Wang E K 1996 J. Electroanal. Chem. 416 105
[31]	Tu C K, Chen K, Tian W D, Ma Y Q 2013 Macromol. Rapid Comm. 34 1237
[32]	Ding H M, Ma Y Q 2012 Biomaterials 33 5798

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