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提出了一种用于评估植物生长状况及环境监测的激光诱导叶绿素荧光寿命测量方法. 采用波长355 nm的激光作为光源激发叶绿素荧光, 由光电倍增管接收其荧光信号, 由于被测叶绿素荧光衰减函数与激光脉冲、仪器响应函数卷积在一起, 根据它们的特性, 运用时间分辨测量法分别测得叶绿素荧光及其背景信号, 并结合一种新型解卷积算法可分离出真实的叶绿素荧光衰减函数, 从而获取叶绿素的荧光寿命. 测试结果表明: 该方法能够实现叶绿素荧光寿命的高精度实时监测, 对不同叶绿素含量的溶液荧光寿命进行了测试, 证明叶绿素含量与其荧光寿命具有相关性, 并且拟合了叶绿素含量与荧光寿命的标定曲线.A laser-induced fluorescence lifetime measurement method is presented to evaluate living status for plant growth and the environmental monitoring. A 355 nm laser is used as excitation source for exciting chlorophyll fluorescence, and the fluorescence signals are received by a photomultiplier. Because the measured signal is the convolution of the reliable fluorescence decay signals, laser pulse and instrument response function, according to their characteristics, the time-resolved measurement method is used to estimate the chlorophyll fluorescence and background signals separately; the real fluorescence decay signals are separated by combining a novel deconvolution method, and the chlorophyll fluorescence lifetime can be retrieved. Experiment shows that the method proves to be a high accuracy and real-time monitoring chlorophyll fluorescence lifetime technique; and the chlorophyll solution fluorescence lifetime is measured for different concentrations. The result can prove that the chlorophyll concentration is related to its fluorescence lifetime, and the calibration curves of chlorophyll concentration and fluorescence lifetime is fitted.
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Keywords:
- fluorescence lifetime /
- laser-induced fluorescence /
- time-resolved measurement /
- chlorophyll concentration
[1] Janusauskaite D, Feiziene D 2012 Acta Agr. Scand. B-S. P. 62 7
[2] Du G Y, Li W T, Li H B, Chung I K 2012 Journal of Plant Biology 55 159
[3] Zhao M, Peng L L 2010 Optics Letters 35 2910
[4] Eva F S 2012 Journal of Luminescence 32 2059
[5] Sun Y W, Qu J L, Zhao L L, Xu G X, Ding Z H, Niu H B 2008 Acta Phys. Sin. 57 772 (in Chinese) [孙怡雯, 屈军乐, 赵羚伶, 许改霞, 丁志华, 牛憨笨 2008 57 772]
[6] Yeh Y C, Yuan C T, Kang C C, Chou P T, Tang J 2008 Applied Physics Letters 93 223110
[7] Tol V, Verhoef R 2009 Agricultural and Forest Meteorology 6 96
[8] Shrestha S, Applegate B E, Park J, Xiao X D, Pande P, Jo J A 2010 Optics Letters 35 2558
[9] Irishina N, Moscoso M, Carminati R 2012 Optics Letters 37 951
[10] García M, Vázquez R, Isakina S, López R 2012 Electrical Communications and Computers 27 317
[11] Kumar A T N, Raymond S B, Bacskai B J, Boas D A 2008 Optics Letters 33 470
[12] Fang J, Papaioanou T, Marcu L 2004 Journal of Biomedical Optics 9 743
[13] Brand L, Johnson L 2008 Fluorescence Spectroscopy (Netherlands: Elsevier Academic Press) pp 228–351
[14] Wang K, Zeng Y, Ding Z H, Meng J, Shi G H, Zheng Y D 2010 Acta Phys. Sin. 59 2471 (in Chinese) [王凯, 曾焱, 丁志华, 孟婕, 史国华, 张雨东 2010 59 2471]
[15] Chen W J, Lin M S, Jiang H L 1985 Acta Optica Sinica 5 904 (in Chinese) [陈文驹, 林美束, 姜宏丽 1985 光学学报 5 904]
[16] Skala M C 2007 Multiphoton Microscopy Fluorescence Lifetime Imaging and Optical Spectroscopy for the Diagnosis of Neoplasia (Carolina: Duke University)
[17] Miao Z, Li S F, Zhang Q Y 2006 Acta Phys. Sin. 55 4321 (in Chinese) [苗壮, 李善锋, 张庆瑜 2006 55 4321]
[18] Yang Z L, Liu Y Q, Yang Y Q 2012 Acta Phys. Sin. 61 037805 (in Chinese) [杨振岭, 刘玉强, 杨延强 2012 61 037805]
[19] Duan Y L 2012 Chinese Journal of Lasers 39 1 (in Chinese) [段亚丽 2012 中国激光 39 1]
[20] Lichtenthaler H K, Langsdorf G, Lenk S 2005 Photosynthetica 43 355
[21] Fang W H, Li Z W, Li Z L, Qu G N, Ouyang S L, Men Z W 2012 Acta Phys. Sin. 61 153301 (in Chinese) [房文汇, 里佐威, 李占龙, 曲冠男, 欧阳顺利, 门志伟 2012 61 153301]
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[1] Janusauskaite D, Feiziene D 2012 Acta Agr. Scand. B-S. P. 62 7
[2] Du G Y, Li W T, Li H B, Chung I K 2012 Journal of Plant Biology 55 159
[3] Zhao M, Peng L L 2010 Optics Letters 35 2910
[4] Eva F S 2012 Journal of Luminescence 32 2059
[5] Sun Y W, Qu J L, Zhao L L, Xu G X, Ding Z H, Niu H B 2008 Acta Phys. Sin. 57 772 (in Chinese) [孙怡雯, 屈军乐, 赵羚伶, 许改霞, 丁志华, 牛憨笨 2008 57 772]
[6] Yeh Y C, Yuan C T, Kang C C, Chou P T, Tang J 2008 Applied Physics Letters 93 223110
[7] Tol V, Verhoef R 2009 Agricultural and Forest Meteorology 6 96
[8] Shrestha S, Applegate B E, Park J, Xiao X D, Pande P, Jo J A 2010 Optics Letters 35 2558
[9] Irishina N, Moscoso M, Carminati R 2012 Optics Letters 37 951
[10] García M, Vázquez R, Isakina S, López R 2012 Electrical Communications and Computers 27 317
[11] Kumar A T N, Raymond S B, Bacskai B J, Boas D A 2008 Optics Letters 33 470
[12] Fang J, Papaioanou T, Marcu L 2004 Journal of Biomedical Optics 9 743
[13] Brand L, Johnson L 2008 Fluorescence Spectroscopy (Netherlands: Elsevier Academic Press) pp 228–351
[14] Wang K, Zeng Y, Ding Z H, Meng J, Shi G H, Zheng Y D 2010 Acta Phys. Sin. 59 2471 (in Chinese) [王凯, 曾焱, 丁志华, 孟婕, 史国华, 张雨东 2010 59 2471]
[15] Chen W J, Lin M S, Jiang H L 1985 Acta Optica Sinica 5 904 (in Chinese) [陈文驹, 林美束, 姜宏丽 1985 光学学报 5 904]
[16] Skala M C 2007 Multiphoton Microscopy Fluorescence Lifetime Imaging and Optical Spectroscopy for the Diagnosis of Neoplasia (Carolina: Duke University)
[17] Miao Z, Li S F, Zhang Q Y 2006 Acta Phys. Sin. 55 4321 (in Chinese) [苗壮, 李善锋, 张庆瑜 2006 55 4321]
[18] Yang Z L, Liu Y Q, Yang Y Q 2012 Acta Phys. Sin. 61 037805 (in Chinese) [杨振岭, 刘玉强, 杨延强 2012 61 037805]
[19] Duan Y L 2012 Chinese Journal of Lasers 39 1 (in Chinese) [段亚丽 2012 中国激光 39 1]
[20] Lichtenthaler H K, Langsdorf G, Lenk S 2005 Photosynthetica 43 355
[21] Fang W H, Li Z W, Li Z L, Qu G N, Ouyang S L, Men Z W 2012 Acta Phys. Sin. 61 153301 (in Chinese) [房文汇, 里佐威, 李占龙, 曲冠男, 欧阳顺利, 门志伟 2012 61 153301]
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