微生物远红外波段复折射率测定及模型构建

孙杜娟; 胡以华; 顾有林; 王勇; 李乐

doi:10.7498/aps.62.094218

摘要

选择枯草芽孢杆菌、黑曲霉孢子和黑曲霉菌丝体等3种典型微生物制备生物样品, 采用显微红外光谱仪对3种微生物样品在2.5—15 μm波段的红外反射光谱进行测定, 基于Kramers-Kronig (K-K)关系计算得到了微生物样品在6—14 μm波段的复折射率. 以水、蛋白质和核酸的红外吸收特性为基础,根据微生物样品的红外吸收谱线计算了微生物样品含水量. 在3种微生物远红外波段复折射率和含水量的实测数据基础上, 构建了生物颗粒在6—14 μm远红外波段的复折射率模型, 分析了模型的可靠性. 模型的构建对发展生物样品的快速分析、鉴定方法, 进而推动生物气溶胶探测、识别技术进步具有重要意义.

关键词:

Abstract

Bacillus subtilis, Aspergillus niger spore and Aspergillus niger mycelium are chosen for samples preparation. The above three samples' reflection spectra in 2.5—15 μm band are recorded by using infrared microscopic spectrometer. From Kramers-Kronig (K-K) relationship, microbe samples' complex refractive indexes in 6—14 μm band are calculated. According to the microbe samples' absorption spectra, their water contents are calculated using the absorption characteristics of water, protein and nucleic acid. Based on the above three microbe samples' water contents and complex refractive indexes in far infrared band, biologic particle's complex refractive index model in 6—14 μm band are constructed and the model's reliability is analyzed. The construction of biologic particle's complex refractive index model has important significance in the development of rapid analysis and identification method of biologic samples, and the detection and identification technologies of biologic aerosol.

Keywords:

作者及机构信息

1.
脉冲功率激光技术国家重点实验室(电子工程学院), 合肥 230037;

2.
电子制约技术安徽省重点实验室, 合肥 230037

基金项目: 国家自然科学基金(批准号:61271353)资助的课题.

Authors and contacts

1.
State Key Laboratory of Pulsed Power Laser Technology (Electronic Engineering Institute, Hefei 230037, China;

2.
Anhui Province Key Laboratory of Electronic Restriction Technology, Hefei 230037, China

Funds: Project supported by the National Natural Science Foundation of China (Grant No. 61271353).

参考文献

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施引文献

[1]	Nachman P, Chen G, Pinnick R G, Hill S C, Chang R K, Mayo M W, Fernandez G L 1996 Appl. Opt. 35 1069
[2]	Dalterio R A, Nelson W H, Britt D, Sperry J, Psaras D, Tanguay J F, Suid S L 1986 Appl. Spectrosc. 40 86
[3]	Dalterio R A, Nelson W H, Britt D, Sperry J, Tanguay J F, Suid S L 1987 Appl. Spectrosc. 41 234
[4]	Bronk B V, Reinisch L 1993 Appl. Spectrosc. 47 436
[5]	Pinnick R G, Hill S C, Nachman P 1995 Aerosol Science and Technology 23 653
[6]	Feng C X, Huang L H, Zhou G C, Han J, Zeng A J, Zhao Y K, Huang H J 2010 Chinese Journal of Lasers 37 2593 (in Chinese) [冯春霞, 黄立华, 周光超, 韩杰, 曾爱军, 赵永凯, 黄惠杰 2010 中国激光 37 2593]
[7]	Lan T G, Xiong W, Fang Y H, Li D C, Yuan Y M 2010 Acta Optica Sinica 30 1656 (in Chinese) [兰天鸽, 熊伟, 方勇华, 李大成, 袁越明 2010 光学学报 30 1656]
[8]	Tuminello P S, Arakawa E T, Khare B N 1997 Appl. Opt. 36 2818
[9]	Grosse P, Offermann V 1991 Appl. Phys. 52 138
[10]	Booij H C, Thoone G P J M 1982 Rheol. Acta. 21 15
[11]	Bohren C F, Huffman D R 1983 Absorption and scattering of light by small particles. (New York: John Wiley & Sons, Inc.) p228
[12]	Bohren C F, Huffman D R 2009 Principles of Optics Electromagnetic Theory of Propagation, Inference and Diffraction of Light (Beijing: Publishing House of Electronics Industry) p32 (in Chinese)
[13]	Dou Z W, Li X X, Zhao J J 2001 Acta ArmamentarII 32 498
[14]	Rosenheimer M S, Linker R 2009 Journal of Quantitative Spectroscopy ＆ Radiative Transfer 110 1147
[15]	Ross K F A, Billinge E 1957 J. Gen. Microbiol. 16 418
[16]	Ci Y X, Zang K S, Gao T Y 2002 Chemical Journal of Chinese Universities 23 1047 (in Chinese) [慈云祥, 臧凯赛, 高体玉 2002 高等学校化学学报 23 1047]
[17]	Li J W, Si M Z 2009 Chinese Journal of Spectroscopy Laboratory 26 888 (in Chinese) [李家旺, 司民真 2009 光谱实验室 26 888]

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