The design of an efficient one-way Hash function with good performance is a hot spot in modern cryptography researches. In this paper, a Hash function construction method based on two-dimensional hyper-chaotic mappings is proposed. The initial values of the two-dimensional hyper-chaotic system come from the chaotic sequence, produced by a series of operations including chaotic mappings and linear transformation based on the plain text, and then modulates the signals resulting from the iteration of the hyper-chaotic system to construct a one-way Hash function. Simulation and analysis demonstrate that the new method has the merit of convenience, high sensitivity to initial values, good Hash performance, weak collision property, and better security.