As a new two-dimensional material, bismuth nanosheet is an effective modulator for realizing a mid-infrared pulsed laser, which benefits from its suitable band gap, higher carrier mobility and better room temperature stability, as well as its excellent electrical and optical properties. The mid-infrared single-crystal fiber is a preferable gain medium for high-power laser because of its advantages of both crystal and fiber. In this paper, a bismuth nanosheet saturable absorber is successfully prepared by the ultrasonic method and used for the first time in a diode-pumped Er:CaF₂ single-crystal fiber mid-infrared passively Q-switching pulsed laser. A compact concave planar linear resonator is designed to study the Q-switching Er:CaF₂ single-crystal fiber laser with bismuth nanosheets serving as saturable absorbers. The pump source is a fiber-coupled semiconductor laser with a core diameter of 105 μm, a numerical aperture of 0.22, and a central emission wavelength of 976 nm. The pump light is focused onto the front end of the gain medium through a coupled collimating system with a coupling ratio of 1∶2. The gain medium is a 4 at.% Er³⁺:CaF₂ single-crystal fiber grown by the temperature gradient method, and this fiber has two polished but not coated ends, a diameter of 1.9 mm, and a length of 10 mm. To reduce the thermal effect, the single-crystal fiber is tightly wrapped with indium foil and mounted on a copper block with a constant temperature of 12 ℃. The input mirror has a high reflection coating at 2.7–2.95 μm and an antireflection coating at 974 nm, with a curvature radius of 100 mm. A group of partially transmitting plane mirrors are used as output couplers, respectively, with transmittances of 1%, 3%, and 5% at 2.7–2.95 μm. The total length of the resonant cavity is 26 mm. By inserting the bismuth nanosheet into the resonator and carefully adjusting its position and angle, a stable mid-infrared Q-switching laser is obtained. At the absorbed pump power of 1.52 W, a pulsed laser with an average output power of 190 mW is obtained for an output mirror with a transmittance of 3%. The shortest pulse width is 607 ns, the repetition frequency is 58.51 kHz, and the corresponding single pulse energy and peak power are 3.25 μJ and 5.35 W, respectively.