The polycrystalline samples of La0.45Ca0.55Mn1-xVxO3(x=0.00, 0.10) have been prepared by the solid-phase reaction. Efects of V5+ substitution for Mn3+/Mn4+ on charge ordering and spin-glass state are studied by X-ray diffraction spectrum, temperature dependence of magnetization, and electron spin resonance spectra. The results indicate that charge ordering of the original system is almost destroyed, and spin-glass state at about 40 K is melted by 10% of V substitution for Mn. The charge ordering phase is destroyed mainly because of V5+ ions substitution for Mn3+/Mn4+, which increases the ratio of Mn3+ to Mn4+ and so causes ferromagnetic double-exchange to be superior to antiferromagnetic super-exchange. In addition, the spin-glass state is melted because V substitution for Mn destroys the formation condition of spin-glass state that a small quantity of ferromagnetic components exist under the antiferromagnetic backgrornd.