In this paper, based on the equivalent transmission line model a method of designing left-handed metamaterials micro-structures is proposed, verified and analyzed. Firstly, according to the basic transmission line model with left and right-handed characteristics, we build a new left-handed model by analogy. Then according to the qualitative analysis of the corresponding relationship of each component in the transmission line network to the shape and size of continuous medium micro-structure configuration, the specific size of model is inferred, and thus a reasonable left-handed metamaterials micro-structure configuration and the corresponding transmission line model are established. We have conducted a series of tests including numerical simulation, which verifies that there does exist the left-handed characteristic in a certain frequency band. Finally based on the numerical simulation and experiment the effects each micro-structural parameter on left-handed frequency position and bandwidth are analyzed, and the size parameter that affects the left hand characteristic most seriously is determined.