A series of chalcogenide glasses based on Ge25Ga10Se65 system co-doped with Ho3+/Pr3+ ions of different ratios were synthesized by melt-quenching technique. The absorption spectra,mid-infrared fluorescence and lifetime of glass samples under 908 nm laser excitation were measured. The emission cross section of Ho3+:5I7→5I8 and absorption cross section of Pr3+:3H4→3F2 were calculated. The energy transfer efficiency between Ho3+ and Pr3+ ions with different Pr3+ ion concentrations have been discussed. Through fitting the 2.0 μm fluorescence decay curves of Ho3+ ions,the energy transfer regime was judged. The results prove thatTm3+ is an efficient sensitizer which enhances the Ho3+: 2.9 μm fluorescence intensity significantly.