The fluorescence spectra of the ethanol-water mixture excited by ultraviolet lig ht and the excitation spectra corresponding to the fluorescence peaks are inves tigated respectively. It is found that the mixtures emit fluorescence photons, which is different from pure ethanol and water. One can conclude that new molec ule clusters are created by mixing of the ethanol and water molecules. The inci dent light of different wavelengths irradiates the solution and the fluorescen ce quantum yield at each wavelength is calculated firstly, and then the excited light with the maximum quantum yield is selected to irradiate the mixture. By the experimental results of fluorescence spectra of solution with different con centrations, the possible structures of these new molecule clusters are discuss ed. The general absorptivity and fluorescence counts are calculated basing on t he additive property of absorptivity and fluorescence intensity, elucidating the fluorescence spectra characteristic of ethanol-water mixture. The research may contribute to the study of the new molecule clusters of ethanol-water.