We studied the c-axis magnetoresistance (H∥ab,J⊥c) of a typical layered perovskite superconducting single crystals Sr2RuO4.It is shown that the magnetoresistance is anisotropic and strongly temperature dependent.When rotating the direction of magnetic field in the ab-plane, the magnetoresistance varies periodically, and the maximum appears as the applied magnetic field is in the (110) direction.We analyze this transport property with respect to the Fermi surface anisotropy,the quasiparticle scattering rate anisotropy and the band dispersion anisotropy, respectively.