There are unidirectional anisotropy in 81 NiFe/Cr multilayers prepared by electronic beam evaporated, The samples exhibit asymmetrical hysteresis loop. which eguivalent to the existence of an exchange bias of the order of 1 Oe. Two branches of magnetoresistance loop measured with increasing and decreasing magnetic field respectively are different especially in transvers field condition. The maximum value of transvers magnetoresistance of some samples reaches 0.78% and with maximum anisotropy magnetoresistance （Ｒｍａｘ－Ｒｍｉｎ）／Ｒ of 4.72%.That value is more larger than the value 3%１］ of 82 NiFe alloy with high quality. We consider that the ma-gnetoresistance effect of these multilayer not only caused by spin-orbit coupling ma-chanism but also under the influence of spin dependent scattering of conduction electron machanism.