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We propose a novel wide-field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination in this article. A new type of polarization filtering image enhancement technology, based on the differences in polarization characteristic between excitation light and fluorescence, is used to filter out the excitation light and enhance image quality. The dynamic speckle illumination is employed to achieve the wide field fluorescence sectioning which has many advantages, such as simple configuration, low cost, rapid response and easy operation. The experimental results indicate that our filtering method can be utilized to improve the image quality significantly, and the dynamic using speckle illumination can be employed to achieve optical sectioning with a wide field and a high vertical resolution capability. Our study is not only to enrich the technological method to extract the weak fluorescence signal from the strong excitation light, but also to provide a significant reference for developing a light wavelength tunable multi-spectral fluorescence microscope with rapid response.
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Keywords:
- laser speckle /
- polarization filtering /
- fluorescence microscope /
- optical sectioning
[1] Wilson T, Shepard C J 1984 Theory and Practice of Scanning Optical Microscopy (New York: Academic Press)
[2] Denk W, Strickler J H, Webb W W 1990 Science 248 73
[3] Somekh M G, See C W, Goh J 2000 Opt. Commun. 2 75
[4] Braeckmans K, Peeters L, Sanders N N, De Smedt 2003 Bio. Phys. J. 85 2240
[5] Ventalon C, Mertz J 2005 Opt. Lett. 30 3350
[6] Ventalon C, Mertz J 2006 Opt. Express 14 7198
[7] Lin H M, Shao Y H, Qu J L, Yin J, Chen S P, Niu H B 2008 Acta Phys. Sin. 57 7641 (in Chinese) [林浩铭, 邵永红, 屈军乐, 尹君, 陈思平, 牛憨笨 2008 57 7641]
[8] Shao Y H, Li H, Wen Q, Wang Y, Qu J L, Niu H B 2010 Chin. Opt. Lett. 10 944
[9] Lim D, Ford T N, Chu K K, Mertz J 2011 J. Biomed. Opt. 16 016014
[10] Mazzaferri J, Kunik D, Belisle J M, Singh K, Lefrancois S, Costantino S 2011 Opt. Express 19 14508
[11] Wen Q, Qu J L, Shao Y H, Wang K G, Niu H B China Patent 201010616211. X [2010-12-30]
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[1] Wilson T, Shepard C J 1984 Theory and Practice of Scanning Optical Microscopy (New York: Academic Press)
[2] Denk W, Strickler J H, Webb W W 1990 Science 248 73
[3] Somekh M G, See C W, Goh J 2000 Opt. Commun. 2 75
[4] Braeckmans K, Peeters L, Sanders N N, De Smedt 2003 Bio. Phys. J. 85 2240
[5] Ventalon C, Mertz J 2005 Opt. Lett. 30 3350
[6] Ventalon C, Mertz J 2006 Opt. Express 14 7198
[7] Lin H M, Shao Y H, Qu J L, Yin J, Chen S P, Niu H B 2008 Acta Phys. Sin. 57 7641 (in Chinese) [林浩铭, 邵永红, 屈军乐, 尹君, 陈思平, 牛憨笨 2008 57 7641]
[8] Shao Y H, Li H, Wen Q, Wang Y, Qu J L, Niu H B 2010 Chin. Opt. Lett. 10 944
[9] Lim D, Ford T N, Chu K K, Mertz J 2011 J. Biomed. Opt. 16 016014
[10] Mazzaferri J, Kunik D, Belisle J M, Singh K, Lefrancois S, Costantino S 2011 Opt. Express 19 14508
[11] Wen Q, Qu J L, Shao Y H, Wang K G, Niu H B China Patent 201010616211. X [2010-12-30]
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