高质量等曲率物参光像面数字全息显微系统

王华英; 刘飞飞; 宋修法; 廖薇; 赵宝群; 于梦杰; 刘佐强

doi:10.7498/aps.62.024207

摘要

对等曲率物参光像面数字全息显微成像系统进行研究,分析了光路配置方法,推导了系统的点扩散函数, 并由此指出了决定系统成像分辨率的因素及系统的成像特点,最后讨论了再现像光场一次位相畸变校正的方法. 结果表明,等曲率物参光像面数字全息具有最大的信息容量;该系统的成像分辨率取决于显微物镜的数值孔径和CCD的像元大小,与CCD的光敏面尺寸无关;物体各点中通过显微物镜的所有频率成分均能被系统完全记录与再现, 样品被照亮区域的大小对记录条件和再现像质没有影响;等曲率物参光像面数字全息系统是一种优化的全息记录与再现系统, 利用该系统可以实现高质量成像.实验结果验证了理论分析的正确性.

关键词:

Abstract

The digital image-plane holographic microscopy which has the same curvature radius in the object light path and the reference light path is studied for the first time to our knowledge. The configuration of this setup is analyzed and built. The point spread function of this system is derived. The factors corresponding to the imaging resolution and the imaging characteristics of this system are analyzed. The method to correct the linear phase distortion in the reconstructed optical field is discussed. The results show that this system has the largest information content, and the imaging resolution of the system is dependent on the numerical aperture of the microscope objective (MO) and on the CCD pixel size, but independent of the photosensitive surface size of the CCD. All frequency components of each point in the object wave which has passed through the MO can completely be recorded and reconstructed. And the size of illuminated region of object has no influence on the recording condition and the quality of the reconstructed image. The digital image-plane holographic microscopy with the same curvature radius in the object light path and the reference light path is an optimized holographic recording system, which can achieve high-quality imaging. The experimental results demonstrate the correctness of the theoretical analysis.

Keywords:

作者及机构信息

1.
河北工程大学信息与电子工程学院, 邯郸 056038;

2.
河北工程大学理学院, 邯郸 056038

基金项目: 国家自然科学基金(批准号: 61077001, 61144005)、河北省自然科学基金(批准号: F2008000750, F2010001038)、河北省科技支撑计划(批准号: 09277101D) 和河北省教育厅科技计划重点项目(批准号: ZH2011241)资助的课题.

Authors and contacts

1.
Scholl of Information & Electrical Engineering, Hebei University of Engineering, Handan 056038, China;

2.
College of Science, Hebei University of Engineering, Handan 056038, China

Funds: Project supported by the National Natural Science Foundation of China (Grant Nos. 61077001, 61144005), the Natural Science Foundation of Hebei Province, China (Grant Nos. F2008000750, F2010001038), the Key Technology Research and Development Program of Hebei Province (Grant No. 09277101D, ZH2011241).

参考文献

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施引文献

[1]	Kemper B, Bally G V 2008 Appl. Opt. 47 A52
[2]	Anand A, Chhaniwal V K, Javidi B 2010 J. Dis. Tech. 6 500
[3]	Miccio L, Finizio A, Puglisi R 2011 Bio.Opt. Exp. 2 331
[4]	Rajshekhar G, Gorthi S S, Rastogi P 2010 Opt. Exp. 18 18041
[5]	Olfatnia M, Singh V R, Xu T, Miao J M, Ong L S 2010 J. Micromech. Microeng 20 085013
[6]	Wang L, Zhao J L, Di J L, Jiang H Z 2011 Opt. Lett. 36 2710
[7]	Wang Y X, Wang D Y, Zhao J, Yang Y S, Xiao X Q, Cui H Q 2011 Chi. Opt. Lett. 9 030901
[8]	Ding S H, Li Q, Li Y D, Wang Q 2011 Opt. Lett. 36 1993
[9]	Zhao J L, Jiang H Z, Di J L 2012 Biomedical Optics and 3D Imaging OSA DSu3C. 1
[10]	Barth R, Staier F, Simpson T, Mittler S, Eisebitt S, Grunze M 2010 J. Biotechnol. 149 238
[11]	Li J C, Fan Z B, Patrice T, Song Q H, Pascal P 2011 Acta. Phys. Sin. 60 034204 (in Chinese) [李俊昌, 樊则宾, Patrice T, 宋庆和, Pascal P 2011 60 034204]
[12]	Hu H F, Wang X L, Li Z L, Zhang N, Zhai H C 2009 Acta. Phys. Sin. 58 7662 (in Chinese) [胡浩丰, 王晓雷, 李智磊, 张楠, 翟宏琛 2009 58 7662]
[13]	Hu H F, Wang X L, Gguo W G, Zhai H C, Wang P 2011 Acta. Phys. Sin. 60 017901 (in Chinese) [胡浩丰, 王晓雷, 郭文刚, 翟宏琛, 王攀 2011 60 017901]
[14]	Wang H Y, Zhao B Q, Song X F 2009 Acta Optica Sinica 29 374 (in Chinese) [王华英, 赵宝群, 宋修法 2009 光学学报 29 374]
[15]	Wang H Y, Guo Z J, Zhang Z H, Liu F F 2012 J. Optoelectronics · Laser 39 0209002 (in Chinese) [王华英, 郭中甲, 张志会, 刘飞飞 2012 中国激光 39 0209002]
[16]	Park Y K, Popescu G, Badizadegan K 2006 Opt. Exp. 14 8263
[17]	IkedaLiu T 2005 Opt. Lett. 30 1165
[18]	Kim M K 2010 SPIE Reviews 1 018005
[19]	Colomb T, Cuche E, Charriére F 2006 Appl. Opt. 45 851
[20]	Cui H K, Wang D Y, Wang Y X, Liu C G, Zhao J, Li Y 2011 Acta. Phys. Sin. 60 044201 (in Chinese) [崔华坤, 王大勇, 王云新, 刘长庚, 赵洁, 李艳 2011 60 044201]

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